analysis/cpout/masks: contains single-channel segmentation masks in 16-bit.tiff images to the analysis/cpout/probabilities folder.Īfter image segmentation the following files have been generated: The downscaled pixel probability images are written out as 16-bit, 3 channel.tiff images to the analysis/cpout/masks folder. The segmentation masks are written out as 16-bit, single-channel.The segmentation masks are converted to 16-bit images.The IdentifySecondaryObjects module expands from the identified nuclei to the border of the full cell probability generated in step 3 or until touching the neighboring cell.The MeasureObjectSizeShape module measures the size of the nuclei and the FilterObjects module filters nuclei below a specified threshold.The advanced settings can be adjusted to improve segmentation. Use the test mode and enable the "eye" icon next to the module to observe if nuclei are correctly segmented. The IdentifyPrimaryObjects module is crucial to correctly identifying nuclei.This step can be adjusted or removed to increase segmentation success. The nuclear probabilities are smoothed using a gaussian filter.The nulcear and cytoplasmic channels are summed up to form a single channel indicating the full cell probability.In ColorToGray the 3 channel probability images are split into their individual channels: channel 1 - nucleus channel 2 - cytoplasm channel 3 - background.The images containing pixel probabilities are downscaled by a factor of 0.5 to match the initial image dimensions.The files ending with _Probabilities.tiff are read in as part of the NamesAndTypes module.The following steps are part of the pipeline: In the Output Settings adjust the Default Output Folder to analysis/cpout.Drag and drop the analysis/ilastik folder into the Images window.Set up the pipeline by importing the resources/pipelines/2_segment_ilastik.cppipe pipeline into CellProfiler and perform following steps: The project did not identify anything that should discourage further developement of the impregnation system.To segment individual objects (here these are cells) in images, the following CellProfiler pipeline reads in pixel probabilities (generated in Ilastik pixel classification) for segmentation. The conclusion of the project is that the new impregnation system makes composite materials with short-term mechanical properties comparable to the reference system. Analysis of the 24 layer tubes and 4 layer tubes made at different winding speeds indicate that the new impregnation system is capable of manufacturing composites with an average fiber volume fraction of 60.4 \%. This indicates that the composite made with the new system has a high resin volume fraction. Image analysis show that the 4 layer tube made with the new system has a lower fiber volume fraction than the reference tube, and lower void content. The image analysis software CellProfiler was used to make programs to identify fiber volume fraction and void content of the composites. Microscopy and image analysis was conducted on material made by the new system and the reference system. Taken into account the standard deviation of the tests there are grounds for stating that the new impregnation system makes composite material with short-term mechanical properties equal to the reference system. The composite made with the new impregnation system had an average short-beam strength of 37.5 MPa, and the reference strength was 36.5 MPa. The 24 layer tubes were tested in short-beam shear conditions. Results from the split-disk tensile test show that the apparent tensile strength of composite material, with an average of 1860.9 MPa, from the new impregnation system is comparable to the reference material, 1754.5 MPa. The 4 layer tubes were tested in split-disk tensile tests. The tubes were wound with 4 and 24 layers. Two types of composite tubes were wound with the system and with the resin bath system as a reference. The composite quality was evaluated by mechanical testing and microscopy. Winding with the new system was easy and did not lead to any issues. The fibers are only in contact with the PTFE tube and the resin. The impregnation unit has three main parts, two aluminum parts that greates a sinusoidal shape, and a disposable PTFE tube. The impregnation system concist of three main parts, the impregnation unit, resin reservoir and fixtures. A new enclosed impregnation system for the filament winding machine at NTNU has been designed and manufactured.
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